The HOXB4 Antibody (CAB16878) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reacts strongly with human samples, making it suitable for Western blot applications. By binding to the HOXB4 protein, this antibody enables researchers to analyze and detect HOXB4 expression in various cell types, providing crucial insights into the role of HOXB4 in hematopoiesis and cancer development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
HOXB4 Antibody
SKU:
CAB16878
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HOX2, HOX2F, HOX-2.6, HOXB4
Positive Sample:
HL-60, Mouse liver
Cellular Localization:
Centrosome, Nucleoplasm, Nucleus.
Calculated MW:
28kDa
Observed MW:
38kDa
This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Intracellular or ectopic expression of this protein expands hematopoietic stem and progenitor cells in vivo and in vitro, making it a potential candidate for therapeutic stem cell expansion.
Purification Method
Affinity purification
Gene ID
3214
RRID
AB_2769831
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HOXB4 Rabbit pAb (CAB16878) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
