Description
HPV18 E6/Protein E6 Recombinant Protein (N-His)
HPV18 E6/Protein E6 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Val158, with an N-terminal His tag. Predicted molecular weight: 21.02 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Protein E6 is a ~18 kDa protein. Plays a major role in the induction and maintenance of cellular transformation. For research use only.
Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host UBE3A/E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6AP targets several other substrates to degradation via the proteasome including host DLG1 or NFX1, a repressor of human telomerase reverse transcriptase (hTERT).
| Product Name: | HPV18 E6/Protein E6 Recombinant Protein (N-His) |
| SKU: | GXRP05214 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | HPV18 |
| Synonyms: | Protein E6, E6 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Val158 |
| Calculated MW: | 21.02 kDa |
| UniProt: | P06463 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
