Description
HPV52 E7/Protein E7 Recombinant Protein (N-His-SUMO & C-Strep)
HPV52 E7/Protein E7 Recombinant Protein (N-His-SUMO & C-Strep) is a recombinant protein expressed in E. coli, spanning Arg2-Leu99, with an N-terminal His-SUMO tag and a C-terminal Strep tag. Predicted molecular weight: 24.53 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Protein E7 is a ~11 kDa protein. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. For research use only.
Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation.
| Product Name: | HPV52 E7/Protein E7 Recombinant Protein (N-His-SUMO & C-Strep) |
| SKU: | GXRP03936 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | HPV52 |
| Synonyms: | Protein E7, E7 |
| Tag: | N-His-SUMO & C-Strep |
| Expression Host: | E. coli |
| Protein Length: | Arg2-Leu99 |
| Calculated MW: | 24.53 kDa |
| UniProt: | P36831 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
