Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (STPA00079). Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 µM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody (STPA00079) at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:1500 for 30-60 min at RT.
HSP70 Antibody (Acetyl Lys77) is a rabbit polyclonal antibody directed against HSP70 (Acetyl Lys77), offered by Assay Genie for research applications. Reported applications include WB, ELISA, ICC/IF and IHC. Reported reactivity: Human, Mouse and Rat. Purified by Peptide Affinity.
HSP70 proteins are a highly conserved family of 70-kDa molecular chaperones that play a central role in protein folding, stabilization, and transport across nearly all cellular compartments—including the cytosol, nucleus, mitochondria, endoplasmic reticulum, and chloroplasts. These proteins are encoded by a multigene family and are upregulated in response to cellular stress, making them key regulators of proteostasis. The HSP70 Acetylated Lysine 77 Antibody is a powerful tool for detecting HSP70 proteins specifically modified at lysine 77, a post-translational modification (PTM) that may influence chaperone activity, protein-protein interactions, and cellular localization. Acetylation at this site occurs within the highly conserved N-terminal ATP-binding domain, which is essential for HSP70’s conformational cycling and substrate release. This antibody may help researchers to monitor acetylation-specific regulation of HSP70 under stress conditions; investigate the role of lysine 77 acetylation in neurodegenerative diseases, cancer, and aging; or differentiate between modified and unmodified HSP70. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µl. For research use only; not for diagnostic or therapeutic procedures.
