Anti-HSPA1L Antibody (CAB1856)

Antibody Name:	Anti-HSPA1L Antibody
Antibody SKU:	CAB1856
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 342-641 of human HSPA1L (NP_005518.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, THP-1, ES-2, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 342-641 of human HSPA1L (NP_005518.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	STRI PKVQ RLLQ DYFN GRDL NKSI NPDE AVAY GAAV QAAI LMGD KSEK VQDL LLLD VAPL SLGL ETAG GVMT ALIK RNST IPTK QTQI FTTY SDNQ PGVL IQVY EGER AMTK DNNL LGRF DLTG IPPA PRGV PQIE VTFD IDAN GILN VTAT DKST GKVN KITI TNDK GRLS KEEI ERMV LDAE KYKA EDEV QREK IAAK NALE SYAF NMKS VVSD EGLK GKIS ESDK NKIL DKCN ELLS WLEV NQLA EKDE FDHK RKEL EQMC NPII TKLY QGGC TGPA CGTG YVPG RPAT GPTI EEVD
Gene ID:	3305
Uniprot:	P34931
Cellular Location:
Calculated MW:	70kDa
Observed MW:	67kDa

Synonyms:

HSPA1L, HSP70-1L, HSP70-HOM, HSP70T, hum70t

Background:

This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein.

UniProt Protein Function:	HSPA1L: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Not induced by heat shock. Expressed in spermatids. Belongs to the heat shock protein 70 family.
UniProt Protein Details:	Protein type:Heat shock protein; Mitochondrial; Chaperone Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: signalosome; nucleoplasm; mitochondrial matrix; cytosol Molecular Function:protein binding; unfolded protein binding; ATP binding Biological Process: binding of sperm to zona pellucida; protein refolding; response to unfolded protein
NCBI Summary:	This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq, Jul 2008]
UniProt Code:	P34931
NCBI GenInfo Identifier:	23831140
NCBI Gene ID:	3305
NCBI Accession:	P34931.2
UniProt Secondary Accession:	P34931,O75634, Q2HXR3, Q8NE72, Q96QC9, Q9UQM1, A6NNB0 B0UXW8,
UniProt Related Accession:	P34931
Molecular Weight:	641
NCBI Full Name:	Heat shock 70 kDa protein 1-like
NCBI Synonym Full Names:	heat shock 70kDa protein 1-like
NCBI Official Symbol:	HSPA1L
NCBI Official Synonym Symbols:	HSP70T; hum70t; HSP70-1L; HSP70-HOM
NCBI Protein Information:	heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1L; heat shock 70kD protein-like 1; heat shock 10kDa protein 1-like; heat shock 70 kDa protein 1-Hom
UniProt Protein Name:	Heat shock 70 kDa protein 1-like
UniProt Synonym Protein Names:	Heat shock 70 kDa protein 1-Hom; HSP70-Hom
Protein Family:	Heat shock 70 kDa protein
UniProt Gene Name:	HSPA1L
UniProt Entry Name:	HS71L_HUMAN

	Western blot analysis of extracts of various cell lines, using HSPA1L antibody (CAB1856) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using HSPA1L antibody (CAB1856). Blue: DAPI for nuclear staining.

Antibodies

HSPA1L Antibody (PACO09825)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)