Anti-Hsp60 Antibody (CAB0969)

Antibody Name:	Anti-Hsp60 Antibody
Antibody SKU:	CAB0969
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-240 of human Hsp60 (NP_002147.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, K-562, Mouse kidney, Mouse liver, Mouse thymus, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-240 of human Hsp60 (NP_002147.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AKDV KFGA DARA LMLQ GVDL LADA VAVT MGPK GRTV IIEQ SWGS PKVT KDGV TVAK SIDL KDKY KNIG AKLV QDVA NNTN EEAG DGTT TATV LARS IAKE GFEK ISKG ANPV EIRR GVML AVDA VIAE LKKQ SKPV TTPE EIAQ VATI SANG DKEI GNII SDAM KKVG RKGV ITVK DGKT LNDE LEII EGMK FDRG YISP YFIN TSKG QKCE FQ
Gene ID:	3329
Uniprot:	P10809
Cellular Location:	Mitochondrion matrix
Calculated MW:	17kDa/61kDa
Observed MW:	61kDa

Synonyms:

HSPD1, CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, Hsp60

Background:

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

UniProt Protein Function:	HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family.
UniProt Protein Details:	Protein type:Chaperone; Mitochondrial Chromosomal Location of Human Ortholog: 2q33.1 Cellular Component: extracellular space; cell surface; protein complex; mitochondrion; early endosome; coated pit; cytosol; secretory granule; membrane; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; plasma membrane; lipopolysaccharide receptor complex; cyclin-dependent protein kinase activating kinase holoenzyme complex Molecular Function:protein binding; p53 binding; lipopolysaccharide binding; ubiquitin protein ligase binding; chaperone binding; ATPase activity; double-stranded RNA binding; unfolded protein binding; DNA replication origin binding; single-stranded DNA binding; ATP binding Biological Process: B cell proliferation; caspase activation; B cell activation; T cell activation; protein stabilization; viral reproduction; positive regulation of apoptosis; positive regulation of interleukin-12 production; protein maturation; isotype switching to IgG isotypes; positive regulation of interleukin-6 production; B cell cytokine production; positive regulation of interleukin-10 production; response to unfolded protein; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; 'de novo' protein folding; protein refolding; positive regulation of T cell activation; positive regulation of T cell mediated immune response to tumor cell; chaperone-mediated protein complex assembly; positive regulation of interferon-alpha production; negative regulation of apoptosis; positive regulation of macrophage activation Disease: Spastic Paraplegia 13, Autosomal Dominant; Leukodystrophy, Hypomyelinating, 4
NCBI Summary:	This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
UniProt Code:	P10809
NCBI GenInfo Identifier:	129379
NCBI Gene ID:	3329
NCBI Accession:	P10809.2
UniProt Secondary Accession:	P10809,Q38L19, Q9UCR6, B2R5M6, B7Z712,
UniProt Related Accession:	P10809
Molecular Weight:	17,100 Da
NCBI Full Name:	60 kDa heat shock protein, mitochondrial
NCBI Synonym Full Names:	heat shock 60kDa protein 1 (chaperonin)
NCBI Official Symbol:	HSPD1
NCBI Official Synonym Symbols:	HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
NCBI Protein Information:	60 kDa heat shock protein, mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
UniProt Protein Name:	60 kDa heat shock protein, mitochondrial
UniProt Synonym Protein Names:	60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
Protein Family:	60 kDa heat shock protein
UniProt Gene Name:	HSPD1
UniProt Entry Name:	CH60_HUMAN

	Western blot analysis of extracts of various cell lines, using Hsp60 antibody (CAB0969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human colon using Hsp60 Rabbit pAb (CAB0969) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using Hsp60 Rabbit pAb (CAB0969) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using Hsp60 Rabbit pAb (CAB0969) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using Hsp60 antibody (CAB0969). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Hsp60 Antibody (CAB0564)	Human HSP60 ELISA Kit
HSPD1 Antibody (PACO06568)	Human HSP-60/HSPD1 (Heat Shock Protein 60) CLIA Kit (HUES01029)
HSPD1 Antibody (PACO07003)	Human 60 kDa heat shock protein, mitochondrial (HSPD1) ELISA Kit
HSPD1 Antibody (PACO09835)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)