Human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) ELISA Kit
The Human 11-beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11B2) ELISA Kit is a powerful tool for the precise measurement of HSD11B2 levels in human samples including serum, plasma, and cell culture supernatants. This kit ensures accurate and dependable results with its high sensitivity and specificity, making it suitable for a variety of research purposes.HSD11B2 is a key enzyme involved in regulating the levels of active glucocorticoids within cells, playing a crucial role in various physiological processes.
Dysregulation of HSD11B2 has been linked to conditions such as metabolic disorders, inflammation, and stress-related diseases, making it a valuable biomarker for investigating these pathologies and exploring potential therapeutic interventions.Through the use of the Human HSD11B2 ELISA Kit, researchers can gain valuable insights into the role of this enzyme in health and disease, paving the way for the development of novel diagnostic tools and targeted treatments.
Product Name:
Human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) ELISA Kit
Product Code:
HUDL01371
Size:
96 Assays
Target:
HSD11b2
Synonyms:
AME, AME1, HSD11K, HSD2, SDR9C3, Short Chain Dehydrogenase/Reductase Family 9C,Member 3, NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-100
90
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
