Human 5-Hydroxytryptamine Receptor 2B (HTR2B) ELISA Kit
The Human 5-Hydroxytryptamine Receptor 2B (HTR2B) ELISA Kit is specifically designed for the accurate detection of 5-Hydroxytryptamine Receptor 2B levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures reliable and reproducible results, making it an excellent tool for researchers in various fields.5-Hydroxytryptamine Receptor 2B, also known as HTR2B, is a critical protein involved in serotonin signaling pathways, playing a role in various physiological functions including mood regulation, appetite control, and cardiovascular functions. Dysregulation of this receptor has been linked to conditions such as depression, obesity, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
This ELISA kit provides researchers with a reliable and efficient method for quantifying 5-Hydroxytryptamine Receptor 2B levels in biological samples, allowing for in-depth analysis and further understanding of its role in various diseases and disorders. With its high sensitivity and specificity, the Human 5-Hydroxytryptamine Receptor 2B (HTR2B) ELISA Kit is an indispensable tool for researchers seeking to uncover the molecular mechanisms underlying serotonin signaling and its implications in human health and disease.
Product Name:
Human 5-Hydroxytryptamine Receptor 2B (HTR2B) ELISA Kit
Product Code:
HUDL01405
Size:
96 Assays
Target:
HTR2B
Synonyms:
Serotonin receptor 2B
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
