Human 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit
The Human 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of HTR2C levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.The HTR2C receptor is a subtype of the serotonin receptor family and plays a crucial role in the central nervous system, regulating mood, appetite, and cognitive function.
Dysregulation of HTR2C has been implicated in various psychiatric and neurological disorders, making it an important biomarker for studying these conditions and identifying potential therapeutic targets.With its high sensitivity and specificity, the Human 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit is a valuable tool for researchers looking to explore the role of HTR2C in health and disease. Trust AssayGenie for accurate and reliable results in your HTR2C research.
Product Name:
Human 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
