Human A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit
The Human A Disintegrin and Metalloprotease 17 (ADAM17) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ADAM17 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.ADAM17 is a key enzyme in cell signaling and is involved in the processing of numerous membrane-bound proteins, including growth factors, cytokines, and cell adhesion molecules. Dysregulation of ADAM17 has been implicated in various diseases, including cancer, inflammation, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Human ADAM17 ELISA Kit offers researchers a powerful tool for investigating the role of ADAM17 in normal physiological processes and disease pathogenesis. Its high sensitivity and specificity ensure accurate measurement of ADAM17 levels, providing valuable insights for further research and drug development efforts.
Product Name:
Human A Disintegrin And Metalloprotease 17 (ADAM17) ELISA Kit
Product Code:
HUDL00059
Size:
96 Assays
Target:
ADAM17
Synonyms:
CD156b, TACE, CSVP, Tumor Necrosis Factor,Alpha,Converting Enzyme, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
