Description
Human A2M Recombinant Protein (N-His)
Human A2M Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser616-Val856, with an N-terminal His tag. Predicted molecular weight: 29.12 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Alpha-2-macroglobulin (A2M) is a ~163 kDa protein. Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. For research use only.
This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
| Product Name: | Human A2M Recombinant Protein (N-His) |
| SKU: | GXRP00387 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CPAMD5, A2M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, Alpha-2-macroglobulin, Alpha-2-M |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser616-Val856 |
| Calculated MW: | 29.12 kDa |
| UniProt: | P01023 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
