Description
Human AATF Recombinant Protein (N-His)
Human AATF Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser273-Ser477, with an N-terminal His tag. Predicted molecular weight: 26.03 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Protein AATF is a ~63 kDa protein. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. For research use only.
During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression.
| Product Name: | Human AATF Recombinant Protein (N-His) |
| SKU: | GXRP03194 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CHE1, AATF, DED, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1, Protein AATF |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser273-Ser477 |
| Calculated MW: | 26.03 kDa |
| UniProt: | Q9NY61 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
