Description
Human ABCB4 Recombinant Protein (N-His)
Human ABCB4 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ile1034-Leu1286, with an N-terminal His tag. Predicted molecular weight: 30.02 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Phosphatidylcholine translocator ABCB4 is a ~141 kDa protein. Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. For research use only.
Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts. Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes. Required for proper phospholipid bile formation.
| Product Name: | Human ABCB4 Recombinant Protein (N-His) |
| SKU: | GXRP01183 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Multidrug resistance protein 3, MDR3, ATP-binding cassette sub-family B member 4, PGY3, Phosphatidylcholine translocator ABCB4, ABCB4, P-glycoprotein 3 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ile1034-Leu1286 |
| Calculated MW: | 30.02 kDa |
| UniProt: | P21439 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
