Human ACE (Angiotensin I Converting Enzyme) CLIA Kit
The Human ACE (Angiotensin I-Converting Enzyme) CLIA Kit is a cutting-edge assay designed for the accurate detection of ACE levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.ACE is a key enzyme involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance in the body. Dysregulation of ACE activity has been linked to various cardiovascular diseases, kidney disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With its advanced technology and precise detection capabilities, the Human ACE CLIA Kit provides researchers with a powerful tool for investigating ACE levels in clinical and experimental settings. Trust AssayGenie to deliver top-quality products for your research needs.
Product Name:
Human ACE (Angiotensin I Converting Enzyme) CLIA Kit
SKU:
HUES00002
Target:
Human ACE (Angiotensin I Converting Enzyme)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACE. You can calculate the concentration of Human ACE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
99-114
83-97
Average (%)
95
105
90
1:4
Range (%)
89-105
95-112
93-107
Average (%)
97
102
99
1:8
Range (%)
91-109
93-106
86-96
Average (%)
99
100
91
1:16
Range (%)
92-108
97-113
87-98
Average (%)
99
104
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
106
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.31
3.86
0.49
1.24
3.71
Standard deviation
0.04
0.12
0.38
0.04
0.09
0.36
C V (%)
7.69
9.16
9.84
8.16
7.26
9.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACE in samples. No significant cross-reactivity or interference between Human ACE and analogues was observed.
