Human ACE (Angiotensin I Converting Enzyme) ELISA Kit
The Assay Genie Human ACE (Angiotensin I Converting Enzyme) ELISA Kit is expertly designed for the precise quantitative analysis of ACE levels in a variety of biological samples. ACE, known as Angiotensin I Converting Enzyme, is a vital enzyme in the renin-angiotensin system, playing a crucial role in regulating blood pressure and maintaining cardiovascular homeostasis. By accurately measuring ACE levels, researchers can gain valuable insights into its involvement in cardiovascular health, hypertension, and renal function, influencing the development of targeted therapeutic interventions in these critical areas. Our ACE ELISA Kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for your research endeavors. Crafted under strict quality control measures, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for conducting in-depth investigations into ACE biology. Trust Assay Genie's ACE ELISA Kit for reliable and precise quantification, empowering your scientific exploration into this essential enzyme.
Product Name:
Human ACE (Angiotensin I Converting Enzyme) ELISA Kit
SKU:
AEES00178
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
