Description
Human ACSS2 Recombinant Protein (N-His)
Human ACSS2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp346-Gln701, with an N-terminal His tag. Predicted molecular weight: 41.98 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Acetyl-coenzyme A synthetase, cytoplasmic (ACSS2) is a ~78 kDa protein. Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. For research use only.
Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity. Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis. Glucose deprivation results in AMPK-mediated phosphorylation of ACSS2 leading to its translocation to the nucleus where it binds to TFEB and locally produces acetyl-CoA for histone acetylation in the promoter regions of TFEB target genes thereby activating their transcription.
| Product Name: | Human ACSS2 Recombinant Protein (N-His) |
| SKU: | GXRP05043 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ACS, AceCS1, ACSS2, Acyl-CoA synthetase short-chain family member 2, Propionate--CoA ligase, Acyl-activating enzyme, Acetate--CoA ligase, ACAS2, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1, AceCS, Acetyl-coenzyme A synthetase, cytoplasmic |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asp346-Gln701 |
| Calculated MW: | 41.98 kDa |
| UniProt: | Q9NR19 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
