The Human ACTN2 (Actinin Alpha 2) CLIA Kit is specifically designed for the precise measurement of actinin alpha 2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an ideal tool for various research applications.Actinin alpha 2 is a vital protein crucial for maintaining the structural integrity of muscle cells and regulating their contraction.
Dysregulation of actinin alpha 2 has been linked to various muscular disorders and cardiovascular diseases, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.For more information on the Human ACTN2 (Actinin Alpha 2) CLIA Kit, please visit www.assaygenie.com/human-actn2-actinin-alpha-2-clia-kit-hues00191/.
Product Name:
Human ACTN2 (Actinin Alpha 2) CLIA Kit
SKU:
HUES00191
Target:
Human ACTN2 (Actinin Alpha 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTN2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTN2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACTN2. You can calculate the concentration of Human ACTN2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
90-102
88-101
Average (%)
101
97
93
1:4
Range (%)
98-114
96-108
94-105
Average (%)
105
102
99
1:8
Range (%)
92-104
94-111
95-109
Average (%)
98
101
103
1:16
Range (%)
86-98
84-98
85-100
Average (%)
92
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
255.15
495.46
1831.61
235.73
520.1
1693.41
Standard deviation
32.07
41.72
152.76
20.58
40.1
187.46
C V (%)
12.57
8.42
8.34
8.73
7.71
11.07
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACTN2 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human ACTN2 in samples. No significant cross-reactivity or interference between Human ACTN2 and analogues was observed.
