The Human ACTN3 (Actinin Alpha-3) CLIA Kit is specifically designed for the accurate detection of Actinin Alpha-3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.Actinin Alpha-3 is a key protein that plays a crucial role in muscle contraction and function. It is also involved in skeletal muscle structure and function, making it a valuable biomarker for studying muscle-related disorders and conditions such as muscle injuries, myopathies, and neuromuscular diseases.
By using the Human ACTN3 CLIA Kit, researchers can easily quantify Actinin Alpha-3 levels in human samples, allowing for better understanding of its role in muscle physiology and pathophysiology. This kit provides a valuable tool for research studies aiming to investigate muscle-related conditions and develop targeted therapies.
Product Name:
Human ACTN3 (Actinin Alpha 3) CLIA Kit
SKU:
HUES00192
Target:
Human ACTN3 (Actinin Alpha 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTN3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTN3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTN3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACTN3. You can calculate the concentration of Human ACTN3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
89-102
100-117
Average (%)
97
96
108
1:4
Range (%)
91-106
85-100
90-102
Average (%)
98
92
95
1:8
Range (%)
84-97
94-110
98-113
Average (%)
90
101
104
1:16
Range (%)
90-105
99-113
99-114
Average (%)
96
107
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
103
EDTA plasma (n=5)
97-109
103
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
253.98
559.86
2190.94
267.68
574.35
2080.57
Standard deviation
22.05
58.56
153.8
27.25
43.82
214.92
C V (%)
8.68
10.46
7.02
10.18
7.63
10.33
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACTN3 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human ACTN3 in samples. No significant cross-reactivity or interference between Human ACTN3 and analogues was observed.
