The Human Adenylate Cyclase 5 (ADCY5) ELISA Kit is a reliable and accurate assay designed for the detection of ADCY5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring robust and reproducible results for a variety of research applications.Adenylate Cyclase 5 is a critical enzyme involved in signal transduction processes, particularly in the production of cyclic AMP (cAMP). Dysregulation of ADCY5 has been linked to various diseases, including metabolic disorders, neurological conditions, and cardiovascular diseases.
By measuring ADCY5 levels, researchers can gain insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets.With its user-friendly protocol and high-performance capabilities, the Human ADCY5 ELISA Kit is an indispensable tool for studying the role of ADCY5 in health and disease. Its accurate quantification of ADCY5 levels provides valuable data for basic research, drug discovery, and clinical studies.
Product Name:
Human Adenylate Cyclase 5 (ADCY5) ELISA Kit
Product Code:
HUDL00075
Size:
96 Assays
Target:
ADCY5
Synonyms:
Adenylate cyclase type V, ATP pyrophosphate-lyase 5
Detection Method:
Competition
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
