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Human ADPN PharmaGenie ELISA Kit (SBRS0003)

SKU:
SBRS0003
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Competitive
€649
Frequently bought together:

Description

Human ADPN PharmaGenie ELISA - Kit Information

Human Adiponutrin (ADPN) is a 53 kD adipokine protein belong to the PNPLA family of phospholipase A2 enzymes. The 481 amino acid human Adiponutrin is a type II transmembrane protein, consisting of the patatin domain (aa 10 - 179) with enzyme activity and a transmembrane segment (aa 42 - 62). Human and mouse adiponutrins share 61% homology in amino acid sequence. Adiponutrin is mainly expressed in adipocytes and serves as a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. Reports have shown that Adiponutrin plays an important role in the balance of energy usage/storage in adipocytes. Its expression level is enhanced in response to insulin. Studies by Dr Clement's group have shown that in obese subjects, short- or long-term calorie restriction diets were associated with a reduction in Adiponutrin gene expression. Adiponutrin mRNA level was previously shown to be negatively correlated with fasting glucose plasma levels and associated with insulin sensitivity of non-diabetic obese and non-obese subjects. Reports from the same group have also shown a strong relationship between Adiponutrin expression, insulin sensitivity and glucose metabolism in human adipose tissue. Other names for Adiponutrin include PNPLA3 (patatin-like phospholipase domain-containing protein 3). Assay Genie Adiponutrin ELISA kit targets the common sequence of human, mouse, and rat. This kit may be used to detect Adiponutrin expression in all these species with high specificity and sensitivity.

Human ADPN PharmaGenie ELISA - Kit Data

Product SKU:SBRS0003
Size:96T
Application:This kit detects both long form (481aa) and short form (477aa) Adiponutrin proteins. No other active isoforms have been reported. Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Uniprot:Q9NST1
Gene ID:80339
Gene Names:ADPN / C22ORF20 / PNPLA3
Synonyms:Patatin-like phospholipase domain-containing protein 3 (EC 3.1.1.3) (Acylglycerol O-acyltransferase) (EC 2.3.1.-) (Adiponutrin) (Calcium-independent phospholipase A2-epsilon) (iPLA2-epsilon)
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.1-1,000 ng/ml
Sensitivity:21 pg/ml
Recommended Dilution:Human: 4X, Mouse: 4X, Rat: 2X
Storage/Stability:Standard, Biotinylated Adiponutrin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Standard Peptide
4.Assay Diluent(s)
5.Biotinylated Peptide
6.HRP-Streptavidin
7.TMB One-Step Substrate
8.Stop Solution
9.Assay Diagram
10.Positive Control Sample
11.Capture Antibody
12.Technical Manual

Other materials and equipment required:

The Assay Genie Human ADPN ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Kit Protocol: