Description
Human AFG3L2 Recombinant Protein (N-His)
Human AFG3L2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys297-Ser765, with an N-terminal His tag. Predicted molecular weight: 54.14 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Mitochondrial inner membrane m-AAA protease component AFG3L2 is a ~88 kDa protein. Catalytic component of the m-AAA protease, a protease that plays a key role in proteostasis of inner mitochondrial membrane proteins, and which is essential for axonal and neuron development. For research use only.
AFG3L2 possesses both ATPase and protease activities: the ATPase activity is required to unfold substrates, threading them into the internal proteolytic cavity for hydrolysis into small peptide fragments. The m-AAA protease carries out quality control in the inner membrane of the mitochondria by mediating degradation of mistranslated or misfolded polypeptides. The m-AAA protease complex also promotes the processing and maturation of mitochondrial proteins, such as MRPL32/bL32m, PINK1 and SP7. Mediates protein maturation of the mitochondrial ribosomal subunit MRPL32/bL32m by catalyzing the cleavage of the presequence of MRPL32/bL32m prior to assembly into the mitochondrial ribosome.
| Product Name: | Human AFG3L2 Recombinant Protein (N-His) |
| SKU: | GXRP04276 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | AFG3-like protein 2, AFG3L2, Paraplegin-like protein |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys297-Ser765 |
| Calculated MW: | 54.14 kDa |
| UniProt: | Q9Y4W6 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
