The Human AGC (Aggrecan) CLIA Kit is specifically designed for the precise measurement of Aggrecan levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it an essential tool for a variety of research applications.Aggrecan is a key component of cartilage tissue, playing a vital role in maintaining cartilage integrity and function. Changes in Aggrecan levels have been implicated in various musculoskeletal disorders, such as osteoarthritis, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Human AGC CLIA Kit offers researchers a comprehensive solution for studying Aggrecan levels in human samples, contributing to a better understanding of cartilage biology and the development of novel treatments for musculoskeletal diseases.
Product Name:
Human AGC (Aggrecan) CLIA Kit
SKU:
HUES00215
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
