Description
Human AGO2 Recombinant Protein (N-His)
Human AGO2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys440-Leu578, with an N-terminal His tag. Predicted molecular weight: 17.74 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Protein argonaute-2 is a ~97 kDa protein. Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). For research use only.
The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2.
| Product Name: | Human AGO2 Recombinant Protein (N-His) |
| SKU: | GXRP10752 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | AGO2, Argonaute RISC catalytic component 2, Argonaute2, EC:3.1.26.n2, EIF2C2, Eukaryotic translation initiation factor 2C 2, PAZ Piwi domain protein, PPD, Protein argonaute-2, Protein slicer, eIF-2C 2, eIF2C 2, hAgo2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys440-Leu578 |
| Calculated MW: | 17.74 kDa |
| UniProt: | Q9UKV8 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
