The Human AGT (Angiotensinogen) CLIA Kit is a highly accurate and reliable assay designed for the detection of angiotensinogen levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.Angiotensinogen is a key protein involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance. Dysregulation of the renin-angiotensin system has been linked to cardiovascular diseases, kidney disorders, and hypertension, making angiotensinogen a valuable biomarker for understanding and potentially treating these conditions. By using the Human AGT CLIA Kit, researchers can accurately measure angiotensinogen levels in human samples, providing valuable insights into the mechanisms underlying cardiovascular and kidney diseases. This kit is a valuable tool for studying the renin-angiotensin system and developing novel therapeutic strategies for related conditions.
Product Name:
Human AGT (Angiotensinogen) CLIA Kit
SKU:
HUES00220
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
