Description
Human AKT1 Recombinant Protein (N-His)
Human AKT1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Ala480, with an N-terminal His tag. Predicted molecular weight: 60.57 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. RAC-alpha serine/threonine-protein kinase (AKT1) is a ~55 kDa protein. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. For research use only.
This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.
| Product Name: | Human AKT1 Recombinant Protein (N-His) |
| SKU: | GXRP01421 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | RAC-alpha serine/threonine-protein kinase, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene c-Akt, Protein kinase B alpha, Protein kinase B, AKT1, PKB |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Ala480 |
| Calculated MW: | 60.57 kDa |
| UniProt: | P31749 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
