Human Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit
The Human Alkaline Sphingomyelinase (ALK-SMASE) ELISA Kit is specifically designed for the accurate measurement of alkaline sphingomyelinase levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable tool for a variety of research applications.Alkaline sphingomyelinase is a crucial enzyme involved in sphingolipid metabolism, playing a key role in cell signaling and lipid homeostasis. Dysregulation of alkaline sphingomyelinase has been linked to various diseases, including metabolic disorders, neurodegenerative diseases, and cancer.
Therefore, measuring alkaline sphingomyelinase levels can provide valuable insights into disease progression and potential therapeutic interventions.With the Human Alkaline Sphingomyelinase ELISA Kit, researchers can accurately quantify alkaline sphingomyelinase levels in human samples, enabling further investigation into the role of this enzyme in disease pathology and potential therapeutic targets.
Product Name:
Human Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
90-102
96
Heparin plasma (n=5)
91-98
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
