Human Amyloid Beta Precursor Like Protein 1 (APLP1) ELISA Kit
The Human APLP1 (Amyloid Beta Precursor-Like Protein 1) ELISA Kit is specially designed for the precise measurement of APLP1 levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and reliable results, making it an invaluable tool for various research applications.APLP1 is a protein closely related to the amyloid precursor protein (APP) and is involved in neuronal development and function. Dysregulation of APLP1 has been linked to neurodegenerative diseases such as Alzheimer's disease, making it a key target for studying disease mechanisms and potential therapeutic interventions.
This ELISA kit provides researchers with a comprehensive solution for quantifying APLP1 levels in human samples, offering valuable insights into the role of this protein in neurodegenerative disorders and other related conditions. Upgrade your research capabilities with the Human APLP1 ELISA Kit from Assay Genie.
Product Name:
Human Amyloid Beta Precursor Like Protein 1 (APLP1) ELISA Kit
Product Code:
HUDL00205
Size:
96 Assays
Target:
APLP1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
