The Human Angiopoietin 1 (ANG1) ELISA Kit is meticulously engineered for the precise and quantitative detection of Angiopoietin 1 levels in diverse biological samples of human origin. Angiopoietin 1 is a critical factor involved in regulating vascular development, endothelial cell function, and angiogenesis, offering valuable insights into vascular homeostasis and angiogenic processes. Our ELISA kit provides exceptional sensitivity and specificity to ensure accurate and reproducible results in your research endeavors. With stringent adherence to quality control standards, this kit delivers robust performance and user-friendly protocols, making it an indispensable tool for exploring the role of ANG1 in various physiological and pathological conditions. Trust Assay Genie's ELISA kit for dependable quantification and comprehensive analysis of Angiopoietin 1 biomarkers in your studies.
Product Name:
Human ANG1 (Angiopoietin 1) ELISA Kit
SKU:
AEES00149
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
