The Human Angiotensin 1-7 (Ang1-7) ELISA Kit is specially designed to accurately measure Ang1-7 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and consistent results, making it an excellent choice for a variety of research applications.Angiotensin 1-7 is a crucial peptide involved in regulating blood pressure and cardiovascular function. It plays a role in balancing the effects of other members of the renin-angiotensin system, making it a potential target for therapeutic interventions in conditions such as hypertension, heart failure, and kidney disease.
By accurately quantifying Ang1-7 levels, researchers can gain valuable insights into the role of this peptide in various disease states and potentially identify new treatment strategies. The Human Angiotensin 1-7 ELISA Kit is a valuable tool for studying the biology of Ang1-7 and its potential as a biomarker for cardiovascular and renal diseases.
Product Name:
Human Angiotensin 1-7 (Ang1-7) ELISA Kit
Product Code:
HUDL00164
Size:
96 Assays
Target:
Ang1-7
Synonyms:
ANG1, AGP1, AGPT, ANG-I
Detection Method:
Competition
Range:
7.81-500pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
