Human ANGPTL2 (Angiopoietin Like Protein 2) CLIA Kit
The Human ANGPTL2 (Angiopoietin-like protein 2) CLIA Kit is specifically designed for the precise measurement of ANGPTL2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.ANGPTL2 is a key protein involved in inflammation, lipid metabolism, and cardiovascular diseases. Its levels have been linked to various health conditions, making it a valuable biomarker for studying disease progression and developing targeted treatments.
This CLIA kit offers researchers a powerful tool for investigating the role of ANGPTL2 in different diseases and discovering potential therapeutic interventions. Trust in the precision and quality of this kit to advance your research efforts in the field of angiogenesis and related disorders.
Product Name:
Human ANGPTL2 (Angiopoietin Like Protein 2) CLIA Kit
SKU:
HUES00237
Target:
Human ANGPTL2 (Angiopoietin Like Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANGPTL2. You can calculate the concentration of Human ANGPTL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
88-103
88-100
Average (%)
101
95
94
1:4
Range (%)
92-106
97-115
91-104
Average (%)
100
105
96
1:8
Range (%)
91-106
97-111
87-98
Average (%)
98
103
92
1:16
Range (%)
93-106
87-99
91-104
Average (%)
99
92
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
84-97
90
Cell culture media (n=5)
100-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
303.87
506.41
2836.86
286.21
505.07
2730.58
Standard deviation
28.62
56.52
242.55
27.48
44.04
220.36
C V (%)
9.42
11.16
8.55
9.6
8.72
8.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANGPTL2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGPTL2 in samples. No significant cross-reactivity or interference between Human ANGPTL2 and analogues was observed.
