Human Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit
The Human ANKRD1 (Ankyrin Repeat Domain Protein 1) ELISA Kit is a specialized tool for the precise measurement of ANKRD1 levels in human samples, including serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit facilitates accurate and consistent results, making it an invaluable resource for various research endeavors.ANKRD1 is a crucial protein known for its involvement in various cellular processes, including cell growth, differentiation, and gene regulation. Its dysregulation has been implicated in a range of diseases, such as cancer, cardiovascular disorders, and autoimmune conditions, highlighting its significance as a biomarker for disease progression and therapeutic development.
By utilizing the Human ANKRD1 ELISA Kit, researchers can gain deeper insights into the role of ANKRD1 in health and disease, paving the way for innovative strategies in diagnosis, prognosis, and treatment interventions. Trust in this advanced kit to elevate your research and uncover new possibilities in the field of biomedical science.
Product Name:
Human Ankyrin Repeat Domain Protein 1 (ANKRD1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
