The Human Anti-Apolipoprotein Antibody (AAHA) ELISA Kit is a cutting-edge tool for detecting and quantifying levels of anti-apolipoprotein antibodies in human samples. This kit offers exceptional sensitivity and specificity, providing precise and accurate results for research purposes. Apolipoproteins are critical proteins that play a vital role in lipid metabolism and cardiovascular health. They are often targeted by the immune system, leading to the production of anti-apolipoprotein antibodies.
Monitoring these antibodies is crucial for understanding their role in various diseases, including atherosclerosis, cardiovascular disease, and autoimmune disorders. With the Human Anti-Apolipoprotein Antibody (AAHA) ELISA Kit, researchers can study the impact of anti-apolipoprotein antibodies on human health and explore potential therapeutic interventions. This kit is an indispensable tool for advancing research in the field of cardiovascular and autoimmune diseases.
Product Name:
Human Anti-Apolipoprotein Antibody/AAHA ELISA Kit
SKU:
HUFI00348
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antigen
Sensitivity:
1.875 ng/mL
Range:
3.125-200 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-103%
82-99%
86-100%
EDTA Plasma (n = 5)
86-102%
85-90%
82-98%
Heparin Plasma (n = 5)
88-101%
90-100%
82-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-104
95
EDTA Plasma (n = 5)
87-101
94
Heparin Plasma (n = 5)
90-104
96
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Biotin-Labeled Antigen Binding: Add biotin-labeled antigen working solution and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add SABC working solution and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes until color develops.
6
Stop Reaction & Reading: Add stop solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes and collect supernatant.
Plasma
Collect in anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue with PBS or lysis buffer, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells on ice using lysis buffer, centrifuge and collect supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
