Human anti-CarP (anti-carbamylated protein) ELISA Kit
The Human Anti-CARP (Anti-Carbamylated Protein) ELISA Kit is specifically designed for the accurate and reliable detection of anti-carbamylated protein antibodies in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise results for a variety of research applications. Carbamylated proteins are modification products formed by the reaction of proteins with urea-derived cyanate. These modified proteins have been implicated in various autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus.
The detection of anti-carbamylated protein antibodies can provide valuable insights into the pathogenesis of these diseases and aid in the development of novel treatment strategies. By utilizing the Human Anti-CARP ELISA Kit, researchers can accurately quantify anti-carbamylated protein antibodies in human samples, allowing for the investigation of their role in disease development and progression. With its high performance and reliability, this kit is an essential tool for advancing research in the field of autoimmune diseases and exploring new therapeutic approaches.
Product Name:
Human anti-CarP (anti-carbamylated protein) ELISA Kit
SKU:
HUFI04695
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double antigen
Sensitivity:
0.469 ng/mL
Range:
0.78-50 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-103%
83-96%
85-97%
EDTA Plasma (n = 5)
95-105%
90-100%
84-98%
Heparin Plasma (n = 5)
88-104%
82-92%
86-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
94-103
98
EDTA Plasma (n = 5)
86-100
92
Heparin Plasma (n = 5)
89-100
93
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Biotin-Labeled Antigen Binding: Add biotin-labeled antigen working solution and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add SABC working solution and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes until color develops.
6
Stop Reaction & Reading: Add stop solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes and collect supernatant.
Plasma
Collect in anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue with PBS or lysis buffer, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells on ice using lysis buffer, centrifuge and collect supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
