Human anti-LAMA5 (Laminin subunit alpha-5) antibody ELISA kit
The Human Anti-LAMA5 (Laminin Subunit Alpha 5) Antibody ELISA Kit is a cutting-edge tool designed for the accurate detection of LAMA5 levels in human serum, plasma, and cell culture supernatants. This kit is equipped with high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications. Laminin subunit alpha 5, also known as LAMA5, plays a crucial role in cell adhesion and migration, making it a key protein in processes such as embryogenesis, tissue regeneration, and tumor metastasis.
Dysregulation of LAMA5 has been linked to various diseases such as cancer, muscular dystrophy, and kidney disorders, making it an essential biomarker for understanding these conditions and developing potential therapeutic interventions. With the Human Anti-LAMA5 Antibody ELISA Kit, researchers can accurately quantify LAMA5 levels in biological samples, leading to a better understanding of the role of this protein in health and disease. Order your kit today and take your research to the next level.
Product Name:
Human anti-LAMA5 (Laminin subunit alpha-5) antibody ELISA kit
SKU:
AEFI00383
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antigen
Sensitivity:
1.875 ng/mL
Range:
3.125-200 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-105%
84-90%
86-99%
EDTA Plasma (n = 5)
91-101%
83-91%
88-100%
Heparin Plasma (n = 5)
89-105%
84-100%
83-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-102
96
EDTA Plasma (n = 5)
85-102
93
Heparin Plasma (n = 5)
85-96
91
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Biotin-Labeled Antigen Binding: Add biotin-labeled antigen working solution and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add SABC working solution and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes until color develops.
6
Stop Reaction & Reading: Add stop solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes and collect supernatant.
Plasma
Collect in anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue with PBS or lysis buffer, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells on ice using lysis buffer, centrifuge and collect supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
