The Human ANXA1 (Annexin A1) ELISA Kit is specifically designed for the quantitative measurement of annexin A1 levels in human serum, plasma, and cell culture supernatants. This innovative kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications. Annexin A1 is a key protein involved in the regulation of inflammation, cell signaling, and apoptosis. It plays a crucial role in various diseases, including inflammatory disorders, cancer, and autoimmune conditions. As a vital biomarker, studying annexin A1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets. Don't miss out on the opportunity to advance your research with the Human ANXA1 ELISA Kit, a powerful tool for detecting and quantifying annexin A1 levels with precision and efficiency.
Product Name:
Human ANXA1 (Annexin A1) ELISA Kit
SKU:
HUES03491
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
