The Human ANXA5 (Annexin A5) ELISA Kit is specifically designed to measure levels of Annexin A5 in human serum, plasma, and cell culture supernatants with high precision and accuracy. This kit offers exceptional sensitivity and specificity, ensuring consistent and trustworthy results for various research purposes.Annexin A5 is a key protein that plays a crucial role in cell membrane dynamics, apoptosis, and inflammation regulation.
Its involvement in various physiological and pathological processes, including clotting disorders, autoimmune diseases, and cancer, makes it a valuable biomarker for studying these conditions and exploring potential treatment strategies.Overall, the Human ANXA5 (Annexin A5) ELISA Kit is a reliable tool for researchers and clinicians seeking to investigate Annexin A5 levels and its implications in health and disease.
Product Name:
Human ANXA5 (Annexin A5) ELISA Kit
SKU:
HUES01635
Target:
Human ANXA5 (Annexin A5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANXA5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANXA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANXA5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANXA5. You can calculate the concentration of Human ANXA5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
88-101
87-101
Average (%)
99
94
93
1:4
Range (%)
92-105
87-101
81-94
Average (%)
98
92
87
1:8
Range (%)
87-98
79-94
82-97
Average (%)
93
86
89
1:16
Range (%)
87-102
85-98
83-95
Average (%)
94
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
84-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
2.38
6.78
1.15
2.3
7.38
Standard deviation
0.07
0.1
0.21
0.06
0.13
0.24
C V (%)
6.48
4.2
3.1
5.22
5.65
3.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANXA5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANXA5 in samples. No significant cross-reactivity or interference between Human ANXA5 and analogues was observed.
