Description
Human APEH Recombinant Protein (N-His)
Human APEH Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ile458-Ser732, with an N-terminal His tag. Predicted molecular weight: 32.84 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Acylamino-acid-releasing enzyme (APEH) is a ~81 kDa protein. Aminopeptidase involved in the degradation of N(alpha)-acylated peptides. For research use only.
Displays exopeptidase activity toward N-formyl and N-acetyl peptides with a preference for dipeptide substrates. Catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Hydrolyzes N-formylated peptides likely arising from N-terminal proteolytic cleavage of bacterial and mitochondrial proteins.
| Product Name: | Human APEH Recombinant Protein (N-His) |
| SKU: | GXRP07549 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Acylamino-acid-releasing enzyme, AARE, 3.4.19.1, Acyl-peptide hydrolase, APH, Acylaminoacyl-peptidase, Oxidized protein hydrolase, OPH, APEH, D3F15S2, D3S48E, DNF15S2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ile458-Ser732 |
| Calculated MW: | 32.84 kDa |
| UniProt: | P13798 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
