Description
Human APEX1 Recombinant Protein (N-His)
Human APEX1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr61-Leu318, with an N-terminal His tag. Predicted molecular weight: 31.56 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA repair nuclease/redox regulator APEX1 is a ~35 kDa protein. Multifunctional protein that plays a central role in the cellular response to oxidative stress. For research use only.
The two major activities of APEX1 are DNA repair and redox regulation of transcriptional factors. Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Also incises at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules.
| Product Name: | Human APEX1 Recombinant Protein (N-His) |
| SKU: | GXRP04332 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | APX, APE1, APEX nuclease, APEN, Redox factor-1, APE-1, REF1, APEX, APE, Apurinic-apyrimidinic endonuclease 1, HAP1, AP endonuclease 1, APEX1, DNA-(apurinic or apyrimidinic site) endonuclease, REF-1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr61-Leu318 |
| Calculated MW: | 31.56 kDa |
| UniProt: | P27695 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
