Description
Human APLN Recombinant Protein (N-His)
Human APLN Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Phe77, with an N-terminal His tag. Predicted molecular weight: 12.21 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Apelin is a ~8 kDa protein. Peptide hormone that functions as endogenous ligand for the G-protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis. For research use only.
Functions as a balanced agonist activating both G(i) protein pathway and beta-arrestin pathway of APLNR. Downstream G proteins activation, apelin can inhibit cAMP production and activate key intracellular effectors such as ERKs. On the other hand, APLNR activation induces beta-arrestin recruitment to the membrane leading to desensitization and internalization of the receptor. Apelin blunts cardiac hypertrophic induction from APLNR on response to pathological stimuli, but also induces myocardial hypertrophy under normal conditions.
| Product Name: | Human APLN Recombinant Protein (N-His) |
| SKU: | GXRP13566 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | APEL, APJ endogenous ligand, APLN, Apelin, Apelin-13, Apelin-28, Apelin-31, Apelin-36 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Phe77 |
| Calculated MW: | 12.21 kDa |
| UniProt: | Q9ULZ1 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
