The Human APOA1 (Apolipoprotein A1) ELISA Kit is specifically designed for the accurate measurement of apolipoprotein A1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring trustworthy and consistent results for various research purposes.Apolipoprotein A1 is a vital component of high-density lipoprotein (HDL) particles and plays a crucial role in lipid metabolism and cardiovascular health. Abnormal levels of APOA1 have been linked to various cardiovascular diseases, making it an important biomarker for studying and diagnosing these conditions.With its easy-to-use format and reliable performance, the Human APOA1 ELISA Kit is an invaluable tool for researchers and clinicians looking to investigate the role of apolipoprotein A1 in health and disease.
Product Name:
Human ApoA1 (Apolipoprotein A1) ELISA Kit
SKU:
HUES01407
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
