The Human ApoB (Apolipoprotein B) ELISA Kit is meticulously crafted to facilitate the quantitative assessment of Apolipoprotein B (ApoB) levels in a diverse range of biological samples. ApoB, a vital protein component of low-density lipoprotein (LDL) cholesterol particles, is centrally involved in lipid metabolism and cardiovascular health. As a key player in cholesterol transport and atherosclerosis development, ApoB serves as a reliable biomarker for assessing cardiovascular risk factors and associated metabolic disorders. Our ApoB ELISA Kit guarantees exceptional sensitivity and specificity, ensuring precise and reproducible measurement of ApoB concentrations. Manufactured under stringent quality control measures, this kit delivers consistent and robust performance, making it an ideal tool for valuable research insights. Trust in the accuracy and efficiency of Assay Genie's ApoB ELISA Kit for your investigative needs.
Product Name:
Human ApoB (Apolipoprotein B) ELISA Kit
SKU:
AEES00172
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
