The Human AQP-3 (Aquaporin-3) ELISA Kit is a reliable and accurate tool for the quantitative measurement of AQP-3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity to ensure precise and reproducible results, making it suitable for various research applications.Aquaporin-3 is a key protein in the aquaporin family that plays a crucial role in regulating water transport in various tissues, including the skin, kidney, and gastrointestinal tract. Dysregulation of AQP-3 has been linked to various skin disorders, such as psoriasis and atopic dermatitis, as well as conditions like cancer and inflammatory diseases.By using the Human AQP-3 ELISA Kit, researchers can gain valuable insights into the role of AQP-3 in health and disease, paving the way for the development of targeted therapies and diagnostic tools. This kit offers a convenient and efficient solution for studying the functions and dynamics of AQP-3 in different biological systems.
Product Name:
Human AQP-3 (Aquaporin 3) ELISA Kit
SKU:
HUES01693
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
