The Human AQP-4 (Aquaporin-4) ELISA Kit is specifically designed for the accurate measurement of aquaporin-4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results, making it an ideal tool for various research applications.Aquaporin-4 is a vital protein involved in the regulation of water transport in the central nervous system, particularly in the brain and spinal cord. Dysregulation of aquaporin-4 has been linked to various neurological disorders, including multiple sclerosis and neuromyelitis optica, highlighting its significance as a potential biomarker for studying these conditions and developing therapeutic interventions. With its advanced technology and user-friendly features, the Human AQP-4 ELISA Kit provides researchers with a reliable and efficient method for detecting and quantifying aquaporin-4 levels, ultimately advancing our understanding of neurological diseases and facilitating the development of targeted treatments.
Product Name:
Human AQP-4 (Aquaporin 4) ELISA Kit
SKU:
HUES01694
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
