Human Arachidonate-12-Lipoxygenase (ALOX12) ELISA Kit
The Human Arachidonate 12-Lipoxygenase (ALOX12) ELISA Kit is a cutting-edge tool designed for the precise quantification of ALOX12 levels in human samples including serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, delivering accurate and reproducible results that are perfect for a diverse array of research applications.ALOX12 is a key enzyme involved in the metabolism of arachidonic acid, playing a crucial role in various physiological and pathological processes. Dysregulation of ALOX12 has been linked to inflammatory diseases, cancer, and cardiovascular disorders, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions.
With its advanced technology and reliable performance, the Human ALOX12 ELISA Kit is an indispensable tool for researchers seeking to deepen their understanding of ALOX12 function and its implications in human health and disease. Order your kit today and unlock new insights into this critical enzyme's role in biological processes.
Product Name:
Human Arachidonate-12-Lipoxygenase (ALOX12) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
