Human Basic Salivary Proline Rich Protein 4 (PRB4) ELISA Kit
The Human Basic Salivary Proline-Rich Protein 4 (PRB4) ELISA Kit is a cutting-edge tool for precise measurement of PRB4 levels in human saliva samples. This kit offers exceptional sensitivity and specificity, providing researchers with reliable and consistent results for various research applications.PRB4 is a key protein in saliva with implications in oral health and disease. Its levels can indicate conditions such as periodontal disease, oral cancer, and immune responses in the oral cavity.
By accurately quantifying PRB4 levels, researchers can gain valuable insights into the pathogenesis of these conditions and potential therapeutic targets.Overall, the Human Basic Salivary Proline-Rich Protein 4 (PRB4) ELISA Kit is a valuable asset for researchers investigating oral health and its impact on overall well-being. Its dependable performance and ease of use make it an essential tool for advancing scientific understanding in this field.
Product Name:
Human Basic Salivary Proline Rich Protein 4 (PRB4) ELISA Kit
Product Code:
HUDL02389
Size:
96 Assays
Target:
PRB4
Synonyms:
Po, BstNI type basic salivary proline-rich protein 4, Parotid o protein, Salivary proline-rich protein II-1, Proline-rich peptide IB-5
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
