Description
Human BCKDK Recombinant Protein (N-His)
Human BCKDK Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser31-Ile412, with an N-terminal His tag. Predicted molecular weight: 45.65 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Branched-chain alpha-ketoacid dehydrogenase kinase is a ~46 kDa protein. Serine/threonine-protein kinase component of macronutrients metabolism. For research use only.
Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle. Phosphorylates and inactivates mitochondrial BCKDH complex a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Associates with the E2 component of BCKDH complex and phosphorylates BCKDHA on Ser-337, leading to conformational changes that interrupt substrate channeling between E1 and E2 and inactivates the BCKDH complex. Phosphorylates ACLY on Ser-455 in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition.
| Product Name: | Human BCKDK Recombinant Protein (N-His) |
| SKU: | GXRP11709 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | BCKDH kinase, BCKDHKIN, BCKDK, BDK, Branched-chain alpha-ketoacid dehydrogenase kinase, EC:2.7.11.1, EC:2.7.11.4, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser31-Ile412 |
| Calculated MW: | 45.65 kDa |
| UniProt: | O14874 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
