Description
Human BHLHE40 Recombinant Protein (N-His)
Human BHLHE40 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser46-Gly186, with an N-terminal His tag. Predicted molecular weight: 18.31 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Class E basic helix-loop-helix protein 40 (BHLHE40) is a ~45 kDa protein. Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. For research use only.
Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1|BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2.
| Product Name: | Human BHLHE40 Recombinant Protein (N-His) |
| SKU: | GXRP04812 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DEC1, bHLHe40, Differentially expressed in chondrocytes protein 1, Class E basic helix-loop-helix protein 40, Class B basic helix-loop-helix protein 2, STRA13, SHARP2, SHARP-2, bHLHb2, Enhancer-of-split and hairy-related protein 2, BHLHB2, BHLHE40, Stimulated by retinoic acid gene 13 protein |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser46-Gly186 |
| Calculated MW: | 18.31 kDa |
| UniProt: | O14503 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
