Human BMP-10 (Bone Morphogenetic Protein 10) CLIA Kit
The Human BMP-10 (Bone Morphogenetic Protein 10) CLIA Kit is a reliable and accurate tool for detecting BMP-10 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures consistent and precise results, making it suitable for a variety of research applications.BMP-10 is a critical growth factor that plays a key role in bone and cartilage formation, as well as cardiovascular development. Dysregulation of BMP-10 has been linked to various diseases, including skeletal defects and cardiovascular disorders.
Therefore, monitoring BMP-10 levels can provide valuable insights into these conditions and aid in the development of potential treatments.The Human BMP-10 CLIA Kit is easy to use and produces reliable data, making it an essential tool for researchers studying BMP-10 and its implications in health and disease. Order now from Assay Genie to advance your research efforts in the field of bone morphogenetic proteins.
Product Name:
Human BMP-10 (Bone Morphogenetic Protein 10) CLIA Kit
SKU:
HUES00394
Target:
Human BMP-10 (Bone Morphogenetic Protein 10)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BMP-10. You can calculate the concentration of Human BMP-10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
95-111
95-110
Average (%)
100
102
103
1:4
Range (%)
98-113
91-105
87-100
Average (%)
105
97
93
1:8
Range (%)
87-102
87-101
96-109
Average (%)
94
94
101
1:16
Range (%)
92-108
95-108
97-113
Average (%)
100
100
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
98
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
89-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.73
293.47
681.02
83.93
315.96
743.25
Standard deviation
8.89
29.52
72.12
9.53
30.27
80.42
C V (%)
9.69
10.06
10.59
11.35
9.58
10.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BMP-10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BMP-10 in samples. No significant cross-reactivity or interference between Human BMP-10 and analogues was observed.
