Human BNP PharmaGenie ELISA Kit (SBRS0320)
- SKU:
- SBRS0320
- Product type:
- ELISA Kit
- Reactivity:
- Human
- Application:
- ELISA
- ELISA Type:
- Sandwich
Frequently bought together:
Description
system_update_altDatasheet
Human BNP PharmaGenie ELISA - Kit Data
| Product | Sample Types | Method |
| Human BNP PharmaGenie ELISA Kit (SBRS0010) | Cell Culture Supernatants and Serum | Competitive |
| Human BNP PharmaGenie ELISA Kit (SBRS0320) | Cell Culture Supernatants, Plasma and Serum | Sandwich |
| Product SKU: | SBRS0320 |
| Size: | 96T |
| Application: | This ELISA antibody pair detects human BNP. Other species not determined. |
| Uniprot: | P16860 |
| Gene ID: | 4879 |
| Gene Names: | NPPB |
| Synonyms: | Natriuretic peptides B (Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 32 (BNP(1-32)) (BNP-32) BNP(1-30) BNP(1-29) BNP(1-28) BNP(2-31) BNP(3-32) BNP(3-30) BNP(3-29) BNP(4-32) BNP(4-31) BNP(4-30) BNP(4-29) BNP(4-27) BNP(5-32) BNP(5-31) BNP(5-29)] |
| Target Species: | Human |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma and Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 14 pg/ml - 1000 pg/ml |
| Sensitivity: | 14 pg/ml |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
Kit Components:
| 1. | Pre-Coated 96-well Strip Microplate |
| 2. | Wash Buffer |
| 3. | Stop Solution |
| 4. | Assay Diluent(s) |
| 5. | Lyophilized Standard |
| 6. | Biotinylated Detection Antibody |
| 7. | Streptavidin-Conjugated HRP |
| 8. | TMB One-Step Substrate |
Other materials and equipment required:
The Assay Genie Human BNP ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Kit Protocol:
| 1. | Prepare all reagents, samples and standards as instructed in the manual. |
| 2. | Add 100 ul of standard or sample to each well. |
| 3. | Incubate 2.5 h at RT or O/N at 4°C. |
| 4. | Add 100 ul of prepared biotin antibody to each well. |
| 5. | Incubate 1 h at RT. |
| 6. | Add 100 ul of prepared Streptavidin solution to each well. |
| 7. | Incubate 45 min at RT. |
| 8. | Add 100 ul of TMB One-Step Substrate Reagent to each well. |
| 9. | Incubate 30 min at RT. |
| 10. | Add 50 ul of Stop Solution to each well. |
| 11. | Read at 450 nm immediately. |
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