Description
Human BRCA1 Recombinant Protein (N-His)
Human BRCA1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln19-Val83, with an N-terminal His tag. Predicted molecular weight: 9.79 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Breast cancer type 1 susceptibility protein (BRCA1) is a ~207 kDa protein. E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. For research use only.
It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle.
| Product Name: | Human BRCA1 Recombinant Protein (N-His) |
| SKU: | GXRP01532 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | BRCA1, Breast cancer type 1 susceptibility protein, RING-type E3 ubiquitin transferase BRCA1, RING finger protein 53, RNF53 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln19-Val83 |
| Calculated MW: | 9.79 kDa |
| UniProt: | P38398 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
